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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
26/11/2015 |
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Data da última atualização: |
26/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CRAVEIRO, S. R.; TOGAWA, R. C.; INGLIS, P. W.; GRYNBERG, P.; MELO, F. L.; RIBEIRO, Z. M. de A.; RIBEIRO, B. M.; BÁO, S. N.; CASTRO, M. E. B. de. |
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Afiliação: |
SALUANA R. CRAVEIRO; ROBERTO COITI TOGAWA, CENARGEN; PETER W. INGLIS; PRISCILA GRYMBERG, CENARGEN; FERNANDO L. MELO; ZILDA MARIA DE ARAUJO RIBEIRO, CENARGEN; BERGMANN M. RIBEIRO; SÔNIA N. BÁO; MARIA ELITA BATISTA DE CASTRO, CENARGEN. |
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Título: |
The genome sequence of Pseudoplusia includes single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC GENOMICS, jun., v.16, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. Results: The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX ? Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may be derived from an ancestor common to these viruses. The homologous repeats (hrs) are absent from the PsinSNPV genome, which is also the case in ChchNPV and TnSNPV. Two p26 gene homologs (p26a and p26b) were found in the PsinSNPV genome. P26 is thought to be required for optimal virion occlusion in the occlusion bodies (OBs), but its function is not well characterized. The P26 phylogenetic tree suggests that this gene was obtained from three independent acquisition events within the Baculoviridae family. The presence of a signal peptide only in the PsinSNPV p26a/ORF-20 homolog indicates distinct function between the two P26 proteins. Conclusions: PsinSNPV has a genomic sequence apparently different from other baculoviruses sequenced so far. The complete genome sequence of PsinSNPV will provide a valuable resource, contributing to studies on its molecular biology and functional genomics, and will promote the development of this virus as an effective bioinsecticide. MenosBackground: Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. Results: The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX ? Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may be derived from an ancestor common to these viruses. The homologous repeats (hrs) are absent from the PsinSNPV genome, which is also the case in ChchNPV and TnSNPV. Two p26 gene homologs (p26a and p26b) were found in the PsinSNPV ... Mostrar Tudo |
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Palavras-Chave: |
Insiticida biológico. |
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Thesagro: |
Baculovirus; Praga; Pseudoplusia Includens. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134081/1/s12864-015-1323-91.pdf
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Marc: |
LEADER 03035naa a2200265 a 4500
001 2029685
005 2024-04-26
008 2015 bl uuuu u00u1 u #d
100 1 $aCRAVEIRO, S. R.
245 $aThe genome sequence of Pseudoplusia includes single nucleopolyhedrovirus and an analysis of p26 gene evolution in the baculoviruses.$h[electronic resource]
260 $c2015
520 $aBackground: Pseudoplusia includens single nucleopolyhedrovirus (PsinSNPV-IE) is a baculovirus recently identified in our laboratory, with high pathogenicity to the soybean looper, Chrysodeixis includens (Lepidoptera: Noctuidae) (Walker, 1858). In Brazil, the C. includens caterpillar is an emerging pest and has caused significant losses in soybean and cotton crops. The PsinSNPV genome was determined and the phylogeny of the p26 gene within the family Baculoviridae was investigated. Results: The complete genome of PsinSNPV was sequenced (Roche 454 GS FLX ? Titanium platform), annotated and compared with other Alphabaculoviruses, displaying a genome apparently different from other baculoviruses so far sequenced. The circular double-stranded DNA genome is 139,132 bp in length, with a GC content of 39.3 % and contains 141 open reading frames (ORFs). PsinSNPV possesses the 37 conserved baculovirus core genes, 102 genes found in other baculoviruses and 2 unique ORFs. Two baculovirus repeat ORFs (bro) homologs, bro-a (Psin33) and bro-b (Psin69), were identified and compared with Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV) bro genes and showed high similarity, suggesting that these genes may be derived from an ancestor common to these viruses. The homologous repeats (hrs) are absent from the PsinSNPV genome, which is also the case in ChchNPV and TnSNPV. Two p26 gene homologs (p26a and p26b) were found in the PsinSNPV genome. P26 is thought to be required for optimal virion occlusion in the occlusion bodies (OBs), but its function is not well characterized. The P26 phylogenetic tree suggests that this gene was obtained from three independent acquisition events within the Baculoviridae family. The presence of a signal peptide only in the PsinSNPV p26a/ORF-20 homolog indicates distinct function between the two P26 proteins. Conclusions: PsinSNPV has a genomic sequence apparently different from other baculoviruses sequenced so far. The complete genome sequence of PsinSNPV will provide a valuable resource, contributing to studies on its molecular biology and functional genomics, and will promote the development of this virus as an effective bioinsecticide.
650 $aBaculovirus
650 $aPraga
650 $aPseudoplusia Includens
653 $aInsiticida biológico
700 1 $aTOGAWA, R. C.
700 1 $aINGLIS, P. W.
700 1 $aGRYNBERG, P.
700 1 $aMELO, F. L.
700 1 $aRIBEIRO, Z. M. de A.
700 1 $aRIBEIRO, B. M.
700 1 $aBÁO, S. N.
700 1 $aCASTRO, M. E. B. de
773 $tBMC GENOMICS, jun.$gv.16, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/02/2016 |
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Data da última atualização: |
11/08/2017 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
VASCONCELOS, M. J. V. de; CARNEIRO, A. A. |
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Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; ANDREA ALMEIDA CARNEIRO, CNPMS. |
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Título: |
Guia para regulamentação de organismos geneticamente modificados. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2015. |
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Páginas: |
33 p. |
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Série: |
(Embrapa Milho e Sorgo. Documentos, 179). |
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Idioma: |
Português |
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Conteúdo: |
O evento transgênico antes de chegar ao mercado precisa passar por um processo de análise científica que consiste na identificação e caracterização do perigo, na avaliação da exposição e na caracterização dos efeitos do risco. A este processo denominamos de “Regulamentação do evento a ser liberado comercialmente”. Este é um processo complexo, que exige uma grande quantidade de análises para mostrar que estes organismos geneticamente modificados (OGMs) não irão causar nenhum mal a seres humanos, animais, plantas e meio ambiente. Este documento tem como objetivo descrever de forma simplificada as etapas envolvidas em um processo de avaliação de risco de organismos geneticamente modificados, obedecendo aos critérios de análise de risco caso a caso e respeitando o princípio da precaução. |
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Palavras-Chave: |
Transgênico. |
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Thesagro: |
Biossegurança; Biotecnologia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146591/1/doc-179.pdf
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Marc: |
LEADER 01370nam a2200181 a 4500
001 2037835
005 2017-08-11
008 2015 bl uuuu u0uu1 u #d
100 1 $aVASCONCELOS, M. J. V. de
245 $aGuia para regulamentação de organismos geneticamente modificados.$h[electronic resource]
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2015
300 $a33 p.
490 $a(Embrapa Milho e Sorgo. Documentos, 179).
520 $aO evento transgênico antes de chegar ao mercado precisa passar por um processo de análise científica que consiste na identificação e caracterização do perigo, na avaliação da exposição e na caracterização dos efeitos do risco. A este processo denominamos de “Regulamentação do evento a ser liberado comercialmente”. Este é um processo complexo, que exige uma grande quantidade de análises para mostrar que estes organismos geneticamente modificados (OGMs) não irão causar nenhum mal a seres humanos, animais, plantas e meio ambiente. Este documento tem como objetivo descrever de forma simplificada as etapas envolvidas em um processo de avaliação de risco de organismos geneticamente modificados, obedecendo aos critérios de análise de risco caso a caso e respeitando o princípio da precaução.
650 $aBiossegurança
650 $aBiotecnologia
653 $aTransgênico
700 1 $aCARNEIRO, A. A.
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Embrapa Milho e Sorgo (CNPMS) |
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